Protein services

protein services hero

Our protein science teams provide biotechnology research and development services to help our customers create opportunities through innovation.

Our expert team of scientists will work closely with you to design and deliver tailored solutions for your biotechnology research.

 

Our services

Recombinant protein expression

  • Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Bacillus subtilis), yeast (Pichia pastoris) and mammalian (HEK293) expression
  • Regulatory approval and access to fermentation capability for expression of proteins and biological materials in all common microbial species up to 1000L scale
  • Suite of expression vectors, chaperones and strains
  • Parallel expression of protein fusions for purification, detection and solubility
  • Fusion tag removal with HRV3C, TEV and SUMO proteases

Antibody technologies

  • Antibody engineering for production of recombinant antibodies, nanobodies, light chains, chimeric mouse/human antibodies, Fv and Fab antibody fragments, recombinant immune complexes and polymeric IgGs
  • Hybridoma maintenance and cloning
  • Antibody purification and isotyping
  • Epitope mapping using random epitope and peptide libraries
  • Isolation and recombinant expression of antibody genes from hybridomas

Molecular and synthetic biology

  • In-house modular DNA assembly platform
  • Combinatorial gene assembly from libraries of promoters, terminators, and genes
  • Multigene assembly
  • Vector design and construction
  • Metabolic pathway construction
  • Site directed mutagenesis
  • PCR and DNA sequencing
  • Quantitative PCR
  • DNA and RNA extraction
  • Fluorometric high sensitivity DNA and RNA quantification
  • Agarose gel electrophoresis

Processing, extraction and formulation

  • Cell fractionation
  • Fast protein liquid chromatography (FLPC) including affinity chromatography, ion exchange chromatography (IX) and size-exclusion chromatography (SEC)
  • Hydrophobic interaction chromatography (HIC)
  • Protein refolding
  • Desalting
  • Freeze drying
  • Activity-guided fractionation
  • Enzyme immobilisation

Product development

  • Compositional analysis of key biomaterials
  • Assessment of product quality and attributes
  • Assessment of product and process specifications
  • In-silco product safety assessment
  • Product stability and ingredient compatibility assessment
  • Assessment of product’s method of action/function
  • Prototype development

Protein characterisation and analysis

  • Protein electrophoresis (SDS-PAGE)
  • Isoelectric focusing (IEF)
  • Western blotting
  • Protein concentration measurement by bicinchoninic acid, Lowry, Bradford and UV
  • Structural integrity by circular dichroism (CD)
  • Thermal stability by differential scanning calorimetry (NanoDSC)
  • Biomolecular interactions by isothermal calorimetry (ITC)
  • Mass spectroscopy (total mass and peptide mapping)
  • Kinetic analysis of biomolecular interactions using surface plasmon resonance and bio-layer interferometry
  • X-ray crystallography
  • Particle size analysis by light scattering and analytical ultracentrifugation

Chemical analysis

  • Spectroscopy (far UV, near UV, infrared, fluorescence)
  • Gas chromatography (GC-FID, GC-MS)
  • High performance liquid chromatography (HPLC/UPLC-variable wavelength/CAD)
  • Nuclear magnetic resonance (1D and 2D)
  • Mass spectroscopy

Biochemical analysis

  • Enzyme and coupled enzyme assays (colourimetric, UV, fluorescence, luminescence)
  • High performance liquid chromatography
  • ELISA (direct, indirect, sandwich, competition & inhibition) for protein quantitation and study of protein-protein interactions
  • Zeta potential
  • Endotoxin measurement
  • Chemical quantitation assays
  • High performance liquid chromatography (HPLC/UPLC-variable wavelength/CAD)

Cell culture assays

  • Cytotoxicity
  • Anti-inflammatory assays (cytochrome oxidase and lipoxygenase, prostaglandin ELISA suite)
  • Confocal Microscopy
  • Reporter gene assays

Physicochemical analysis

  • In-vitro skin permeation studies (Franz diffusion cell)
  • Ex-vivo intestine permeation studies
  • Transmission electron microscopy (TEM) of protein nanomaterials